Journal:

Article Title: Coordinate Intracellular Expression of Salmonella Genes Induced during Infection

doi:

Figure Lengend Snippet: Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.

Article Snippet: The murine macrophage cell line RAW 264.7 and human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine) (ATCC TIB-71, CCL-23, and CCL-6, respectively) were obtained from the American Type Culture Collection, Rockville, Md., and maintained in minimum essential medium (MEM) supplemented with Earle’s salts, l -glutamine, and 10% heat-inactivated fetal calf serum (FCS) (Life Technologies, Rockville, Md.).

Techniques: Expressing, Cell Culture, Incubation, Standard Deviation

Journal: Biochemical pharmacology

Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

doi: 10.1016/j.bcp.2014.08.025

Figure Lengend Snippet: (A) HepG2, (B) LS180, and (C) LS174T cells transiently transfected with hPXR, CYP3A4-luc, and CMV-Renilla were treated for 24 h with indicated concentrations of rifampicin or MET prior to luciferase assay.

Article Snippet: HepG2 liver carcinoma cells, HEK293T cells, LS180 and LS174T human intestinal epithelial cells (derived from human colorectal adenocarcinoma), were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Transfection, Luciferase

Journal: Biochemical pharmacology

Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

doi: 10.1016/j.bcp.2014.08.025

Figure Lengend Snippet: Rifampicin and MET activities in CYP3A4 promoter luciferase reporter assays

Article Snippet: HepG2 liver carcinoma cells, HEK293T cells, LS180 and LS174T human intestinal epithelial cells (derived from human colorectal adenocarcinoma), were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Luciferase

Journal: Biochemical pharmacology

Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

doi: 10.1016/j.bcp.2014.08.025

Figure Lengend Snippet: (A) HepG2, (B) LS180, and (C) LS174T cells transiently transfected with hPXR, CYP3A4-luc, and CMV-Renilla were treated for 24 h with indicated concentrations of rifampicin or indicated diuretics prior to luciferase assay.

Article Snippet: HepG2 liver carcinoma cells, HEK293T cells, LS180 and LS174T human intestinal epithelial cells (derived from human colorectal adenocarcinoma), were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Transfection, Luciferase

Journal: Biochemical pharmacology

Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

doi: 10.1016/j.bcp.2014.08.025

Figure Lengend Snippet: Real-time PCR analysis of (A) CYP3A4 and (B) MDR1 mRNA expression in LS180 cells and (C) CYP3A4 and (D) MDR1 mRNA expression in LS174T cells after 48-h treatment with different compounds as indicated (*p < 0.05; in the t-test comparisons were made between compound-treated and DMSO-treated samples). Western blot showing CYP3A4 and MDR1 protein levels in (E) LS180 and (F) LS174T cells upon MET treatment. The experiments were repeated two times and the data show one representative experiment. The numbers below the protein bands indicate the relative intensity of the protein bands, with the DMSO treated sample set as “1”. In both (E) and (F), anti-CYP3A4 and anti-MDR1 were used to detect CYP3A4 and MDR1 sequentially. Anti-β-actin was finally used to detect β-actin.

Article Snippet: HepG2 liver carcinoma cells, HEK293T cells, LS180 and LS174T human intestinal epithelial cells (derived from human colorectal adenocarcinoma), were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot